Primer

Part:BBa_K1441019:Design

Designed by: Matthew Brewer   Group: iGEM14_Georgia_State   (2014-10-10)


pGMCSR1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers specific for the alteration of IGEM suffixes. The melting temperatures and annealing temperatures of the primers had to be taken into consideration when designing the primers.


Source

This primer is specific to the Bio-bricks suffix following the pGAPz vector. This primer was designed to remove the stop codon present in the standardized iGEM suffixes. This allows for proper amplification, insertion and expression of the insert into pGAPz vectors.


References